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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already explained. Two further splice variants had been noted but detailed nucleotide sequence info is not however available.
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Blocking peptides are peptides that bind specifically on the goal antibody and block antibody binding. These peptide generally contains the epitope regarded with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the very best effects are...
Antibodies bound to the blocking peptide no more bind on the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK get more info kinases. Distinct cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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